Will Tramadol Show Up In A Drug Test (hair Sample Test)?

Updated

I have just gotten my CDL license and I have taken 2 urine test in the past month, I passed both of them since I don't use drugs. My girlfriend has lupus so she has a couple of different pain relievers which include tramadol. I knew I would be taking DOT physical so I began working out and running again in June. I have experienced 2 knee surgeries as well as breaking my lower left leg so the running made my knee sore one day 6/20-24/2014 not sure the exact day so I took one tramadol. Can I still pass a DOT hair sample test if I only took one pill so long ago?

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